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Ullin-repeats. Each of these repeats are composed of a five-helix structural motif in which the helices C to E are organized as a three-helix bundle that interacts with helices A and B to form the cullin repeat (Zheng et al., 2002b). Specifically the first repeat is responsible for binding the substrate adaptor complex of SkpCullin-based Ubiquitin E3 Ligases3 ofand the F-box protein, while the ot

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Substitutions in the trailer region are shown in Fig. 2. At 44 h

bonsaistar43 (#1139) 18 days ago Technology bi-671800 protocol All http://www.jx1980.com   Discuss  | Add To 
Substitutions in the trailer region are shown in Fig. 2. At 44 h posttransfection, the cells were harvested and lysates were prepared. The cells from one well were processed directly for RNA purification, while the cells from a duplicate well were detergent lysed and digested with micrococcal nuclease to destroy naked RNA before being processed for RNA purification. RNA was analyzed by Northern b

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