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Ansfected with the appropriate M plasmid, and radiolabeled with media containing

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Ansfected with the appropriate M plasmid, and radiolabeled with media containing [35S]methionine (25 Ci/ml) for 16 h. The VLP-containing supernatants were collected, placed over a 15 sucrose cushion, and centrifuged to pellet the VLPs as described (6). VLPs and cell lysates were analyzed by SDS-PAGE and phosphorimaging analysis. NCM Complex Exchange Assay--Radiolabeled wild-type or mutant M prote
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